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High antimicrobial activity, innocuous to humans

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LAE® activity vs. Listeria monocytogenes

Success case: LAE® activity vs. Listeria monocytogenes

It has been proved that LAE®  is active against L. monocytogenes in several tests as well as in its application in continuous production processes.

In vitro activity

LAE® Minimum Inhibitory Concentration (MIC) in vitro against L. monocytogenes is:

µg/mL
Listeria monocytogenes B4/978
Listeria monocytogenes ATCC 1531332

Inoculation and superficial application

In heat-treated meat products, LAE® application as a superficial treatment reduces L. monocytogenes viability.

Result table – Inoculation

Luchansky et al. proved that the superficial application of 30 mg/kg of LAE® in 1,5 kg of ham before being vacum packed reduced L. monocytogenes incidence in 6,5 log ufc/piece in inoculated samples with 7,0 log ufc/piece. And in the case of inoculated samples with 3,0 log ufc/piece of L. monocytogenes, the application of 30 mg/kg of LAE® reduced its presence below the detection limit (1,48 log ufc/piece) during 60 days at 4 ºC.

Ham Inoculation
with 7,0 log ufc/piece
ControlLAE® at 30 mg/kgReduction caused by LAE® at 30 mg/kg
1 day at 4 ºC6,9 log ufc/piece<1,48 log ufc/piece>5,42 log ufc/piece
Ham Inoculation with 3,0 log ufc/pieceControlLAE®at 30 mg/kgRedution caused by LAE® at
30 mg/kg
1 day at 4 ºC3,4 log ufc/piece<1,48 log ufc/piece>1,92 log ufc/piece
60 days at 4 ºC4,7 log ufc/piece<1,48 log ufc/piece>3,22 log ufc/piece

Result table – Superficial application

The ABC Research (USA) laboratory has carried out tests in different sliced cooked ham products and sliced smoked turkey, applying LAE® superficially at approximately 160 mg/kg resulting in a noticeable initial reduction of L. monocytogenes of 1,63 log ufc/g and >1,94 log ufc/g respectively.

Initial countingControlLAE® at 200 mg/KgRedution caused by LAE® at 200 mg/Kg
Sliced cooked ham3,67 log ufc/g2,04 log ufc/g1,63 log ufc/g
Sliced smoked turkey3,04 log ufc/g<1,10 log ufc/g>1,94 log ufc/g

Stopforth et al. indicated that the superficial application of 70 mg/kg of LAE® in 70 g ham cooked slices inoculated with 3,3 log ufc/g of L. monocytogenes, reduces to 1 log ufc/g L. monocytogenes presence.

Request the complete study.

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